cd39 fitc  (Miltenyi Biotec)

Journal: eLife

Article Title: Pinpointing the tumor-specific T cells via TCR clusters

doi: 10.7554/eLife.77274

Figure Lengend Snippet: ( a ) Fraction of CD8 + and CD4 + cells out of all tumor-infiltrating CD3 + T cells. ( b ) Fraction of CD39 + PD-1 + cells consisting of CD8 + and CD4 + TILs. ( c , d ) Clonality (calculated as [1 − Normalized Shannon-Wiener index]) of CD8 + and CD4 + ( c ) doube-positive (DP) and ( d ) non-DP TILs. N = number of tested patients. Data analyzed by paired t-test.

Article Snippet: For FACS of PD1 + CD39 + TIL subpopulations, cells were stained with the following antibodies: CD4-BV510 (RPA-T4), CD8-Alexa-647 (SK1), CD127-APC-Cy7 (IL-7Rα, A019D5), PD-1-BV421 (CD279, EH12.2H7) (BioLegend, Germany), CD25-PE (IL-2Rα, Beckman Coulter, USA, Brea, California), CD39-FITC (eBioA1) (eBioscience, USA, Santa Clara, California).

Techniques:

Journal: eLife

Article Title: Pinpointing the tumor-specific T cells via TCR clusters

doi: 10.7554/eLife.77274

Figure Lengend Snippet: ( a ) Experimental pipeline for evaluating CD39 + PD-1 + T cell clonotypes expansion from bulk melanoma TILs cultivated in different conditions. ( b ) Number of T cell receptor (TCR) clonotypes preferentially expanded in bulk TILs of patient mp34 under various culture conditions compared to classical IL-2 high conditions. ( c , d ) Fraction of regulatory T cells (T reg s) (gated as CD4 + CD25 + CD127 - FoxP3 + ) among CD4 + T cells of patients ( c ) mp42 and ( d ) mp32. ( e , f ) Proportion of CD127 high CD25 low cells among ( e ) CD4 + and ( f ) CD8 + TIL subsets from patient mp41. Data in c, e, f analyzed by one-way ANOVA, Bonferroni’s multiple comparisons test.

Article Snippet: For FACS of PD1 + CD39 + TIL subpopulations, cells were stained with the following antibodies: CD4-BV510 (RPA-T4), CD8-Alexa-647 (SK1), CD127-APC-Cy7 (IL-7Rα, A019D5), PD-1-BV421 (CD279, EH12.2H7) (BioLegend, Germany), CD25-PE (IL-2Rα, Beckman Coulter, USA, Brea, California), CD39-FITC (eBioA1) (eBioscience, USA, Santa Clara, California).

Techniques:

Journal: eLife

Article Title: Pinpointing the tumor-specific T cells via TCR clusters

doi: 10.7554/eLife.77274

Figure Lengend Snippet:

Article Snippet: For FACS of PD1 + CD39 + TIL subpopulations, cells were stained with the following antibodies: CD4-BV510 (RPA-T4), CD8-Alexa-647 (SK1), CD127-APC-Cy7 (IL-7Rα, A019D5), PD-1-BV421 (CD279, EH12.2H7) (BioLegend, Germany), CD25-PE (IL-2Rα, Beckman Coulter, USA, Brea, California), CD39-FITC (eBioA1) (eBioscience, USA, Santa Clara, California).

Techniques: Cell Culture, Recombinant, Lysis, Isolation, Flow Cytometry, RNA HS Assay, Multiplex Assay

Journal: eLife

Article Title: Pinpointing the tumor-specific T cells via TCR clusters

doi: 10.7554/eLife.77274

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal CD39-FITC (eBioA1) , eBioscience, USA, Santa Clara, California , 11-0399-42 , FACS, flow cytometry, 5 μL/test.

Techniques: Cell Culture, Recombinant, Lysis, Isolation, Flow Cytometry, RNA HS Assay, Multiplex Assay

Journal: Clinical and Experimental Immunology

Article Title: Separation of plasma‐derived exosomes into CD3 (+) and CD3 (–) fractions allows for association of immune cell and tumour cell markers with disease activity in HNSCC patients

doi: 10.1111/cei.13113

Figure Lengend Snippet: Generation of CD4(+)CD39(+) T cells by co‐incubation of primary CD4(+) T cells with CD3(+) exosomes. (a) Representative density plots showing % CD4(+)CD39(+) T cells in co‐cultures of CD4(+) T cells with exosomes carrying high or low levels of inhibitory proteins. (b) Box‐plots show increased conversion of conventional CD4(+) T cells to CD39(+) T cells upon co‐incubation with CD3(+) exosomes carrying high or low levels of inhibitory proteins; *P < 0·05; **P < 0·005. [Colour figure can be viewed at http://wileyonlinelibrary.com]

Article Snippet: Following incubation for 20 h, anti‐CD4‐PE antibody (Beckman Coulter) and anti‐CD39‐FITC antibody (eBioscience) and appropriate isotype controls were used to determine the percentages of CD4 + CD39 + T cells by flow cytometry.

Techniques: Incubation